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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
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Radiolabeled oligonucleotides for antisense imaging.

Arun K Iyer1, Jiang He

  • 1Center for Molecular and Functional Imaging, Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94143.

Current Organic Synthesis
|August 9, 2011
PubMed
Summary
This summary is machine-generated.

Radiolabeled antisense oligonucleotides enable non-invasive gene expression imaging (antisense imaging) using PET and SPECT. Overcoming delivery barriers and improving cellular uptake are key for successful in vivo targeting.

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Area of Science:

  • Molecular imaging
  • Molecular biology
  • Radiochemistry

Background:

  • Antisense imaging utilizes radiolabeled oligonucleotides to detect gene expression in vivo.
  • Radiolabeled oligonucleotides can also track their own delivery and biodistribution.
  • Advances in this field offer new non-invasive molecular imaging tools.

Purpose of the Study:

  • To review developments in antisense imaging probes for PET and SPECT.
  • To highlight challenges and strategies in radiolabeling, stability, delivery, and in vivo targeting.

Main Methods:

  • Review of current literature on radiolabeled oligonucleotide probes.
  • Focus on radiolabeling strategies for SPECT and PET isotopes.
  • Discussion of factors influencing in vivo delivery and targeting.

Main Results:

  • Development of radiolabeled oligonucleotides for non-invasive gene expression detection.
  • Identification of significant barriers to in vivo delivery and cellular internalization.
  • Progress in radiolabeling techniques and probe design.

Conclusions:

  • Antisense imaging holds promise for non-invasive molecular imaging and diagnostics.
  • Overcoming in vivo delivery barriers is crucial for clinical translation.
  • Further research is needed to enhance cellular uptake and target hybridization.