Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Transcriptional Switch for Recombinant Protein Expression in Seed Plastids.

Plant biotechnology journal·2026
Same author

Breeding mitochondria in crops.

Nature plants·2026
Same author

Posttranscriptional tuning of gene expression over a large dynamic range in synthetic tobacco chloroplast operons.

The Plant journal : for cell and molecular biology·2024
Same author

Seed plastids: A novel platform for recombinant protein expression.

Plant biotechnology journal·2024
Same author

Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.

Scientific reports·2023
Same author

Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.

Research square·2023

Related Experiment Video

Updated: May 30, 2026

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
08:13

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Published on: October 17, 2017

Transplastomics in Arabidopsis: progress toward developing an efficient method.

Kerry Ann Lutz1, Arun Azhagiri, Pal Maliga

  • 1Waksman Institute of Microbiology Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 9, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method for plastid genome engineering in Arabidopsis, overcoming previous limitations by using diploid embryonic root cultures for stable transformation. This advancement facilitates studying plastid gene function and regulation.

More Related Videos

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
07:28

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Related Experiment Videos

Last Updated: May 30, 2026

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
08:13

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Published on: October 17, 2017

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
07:28

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Published on: October 4, 2021

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Plastid genome engineering is crucial for understanding plant gene function.
  • Existing methods in Nicotiana tabacum use biolistic delivery and homologous recombination.
  • Previous Arabidopsis plastid transformation protocols yielded sterile plants due to polyploid leaf cells.

Purpose of the Study:

  • To develop a method for stable plastid transformation in diploid Arabidopsis tissues.
  • To enable functional analysis of plastid genes and regulatory mechanisms in Arabidopsis.
  • To overcome sterility issues associated with previous plastid transformation protocols.

Main Methods:

  • Established a steroid-inducible expression system for the BABY BOOM transcription factor.
  • Developed a regulated embryogenic root culture system to generate diploid tissues.
  • Adapted biolistic delivery and homologous recombination for plastid transformation in diploid Arabidopsis cells.

Main Results:

  • Successfully generated diploid embryogenic root cultures for plastid transformation.
  • Overcame the polyploidy limitation of leaf tissues, enabling stable transformation.
  • Created a foundation for future studies on plastid gene function and regulation in Arabidopsis.

Conclusions:

  • The developed embryogenic root culture system provides diploid target tissue for robust plastid transformation in Arabidopsis.
  • This breakthrough facilitates in-depth investigation of plastid gene function, post-transcriptional regulation, and gene interactions.
  • Enables comprehensive characterization of plastid biology and its interplay with nuclear genes in a key model plant.