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Updated: May 30, 2026
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
Published on: February 11, 2022
Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum
Christopher P Corbo1, Nidaa A Othman, Michael C Gutkin
1Laboratory for Developmental Brain Research and Neuroplasticity, Department of Biological Sciences, Wagner College, Staten Island, New York 10301, USA.
Abstract:
Cellular composition of the adult zebrafish (Danio rerio) optic tectal cortex was examined in this study. Morphological techniques such as 1 microm thick serial plastic sections stained with osmium tetroxide and toluidine blue, modified rapid Golgi silver impregnation, GFAP immunohistochemistry, confocal microscopy, as well as scanning and transmission electron microscopy were used. Neuronal and glial components are described and the layers of the cortex are revisited. Specific neuronal arrangements as well as unique glial/ependymal cells are described. A three dimensional rendering of the astrocytic fiber arrangement in the marginal zone is presented and a composite drawing summarizes the cellular composition of the optic tectum.
