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Related Experiment Video

Updated: May 30, 2026

The Examination of Peroxidase-Positive Leukocytes in Semen

The Examination of Peroxidase-Positive Leukocytes in Semen

Published on: January 19, 2024

[Semen analysis training and its evaluation].

Wen-Hong Lu¹, Yi-Qun Gu, Hong Li

¹Department of Male Clinical Research, National Research Institute for Family Planning/WHO Collaborating Center for Research in Human Reproduction, Beijing 100081, China. crescent2002@126.com

Zhonghua Nan Ke Xue = National Journal of Andrology

|August 10, 2011

Summary

This summary is machine-generated.

This study demonstrates that standardized training significantly enhances the precision of semen analysis for new laboratory technicians. The developed training and assessment methods are effective for quality control in andrology labs.

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Area of Science:

Andrology
Laboratory Medicine
Reproductive Health

Background:

Standardized semen analysis is crucial for male fertility assessment.
Inconsistent results can arise from inexperienced laboratory personnel.
Effective training protocols are needed to ensure accuracy in andrology laboratories.

Purpose of the Study:

To establish and evaluate training methods for green-hand laboratorians in standard semen analysis.
To assess the effectiveness of these methods for internal quality control in Chinese andrology laboratories.

Main Methods:

Technicians were trained in WHO-recommended standard semen analysis procedures.
Trainee performance in sperm concentration and motility analysis was statistically evaluated.
Bland-Altman analysis assessed consistency between trainee and trainer results.

Main Results:

Post-training coefficients of variation for sperm concentration and motility were low (e.g., 3.38% for low concentration, 6.62% for high motility).
Consistency analysis showed minimal deviation between trainees and trainers (e.g., 4.77% out of interval for concentration, 7.15% for motility).
No significant differences (P > 0.05) were found in sperm concentration and motility results between trainees and trainers.

Conclusions:

Standardized training significantly improves the precision of semen analysis for novice laboratorians.
The developed training and assessment methodologies are effective and feasible for andrology laboratories.
These methods provide a valuable reference for quality control and technician training.