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[Effect of astragaloside against the oxidative damage on endothelial cells]
Kang-ting Ji1, Ji-fei Tang, Jun-de Chai
1Department of Cardiology, Second Hospital Affiliated to Wenzhou Medical College, Zhejiang 325000. jktdtl@sohu.com
Objective:
To observe the effect of astragaloside on oxidative low-density lipoprotein (Ox-LDL) mediated oxidative damage of endothelial progenitor cells (EPCs).
Methods:
Peripheral blood mononuclear cells(PBMCs) were isolated by Ficoll density gradient centrifugation, and EPCs were identified by flow cytometry. Adherent cells were collected after seven-day incubation and randomly divided into the normal control group, the Ox-LDL group (as the model group, at the dose of 100 microg/mL), the low, middle, and high astragaloside groups (with 100 microg/mL Ox-LDL plus 2, 10, and 50 microg/mL astragaloside). Twenty-four h later, the proliferation and adhesion capabilities of EPCs were observed using MTT colorimetry and the adhesion capability detection. Levels of superoxide dismutase (SOD) and malonaldehyde (MDA) in the cell supernate of each group were determined.
Results:
After Ox-LDL damage, the proliferative and adhesive capacities of EPCs were significantly injured (53 +/- 8 vs 42 +/- 6, 0.49 +/- 0.12 vs 0.37 +/- 0.02, both P<0.05). The SOD content obviously decreased (21.95 +/- 1.43 vs 14.76 +/- 3.99, P<0.01), the MDA content obviously increased (3.72 +/- 0.30 vs 5.57 +/- 0.64, P<0.01). After intervened by astragaloside for 24 h, the proliferative and adhesive capacities of EPCs were significantly improved. The SOD contents of astragaloside intervention groups were obviously improved and the MDA content obviously lowered.
Conclusions:
Astragaloside showed significant protection on Ox-LDL damaged EPCs. Its mechanism might be correlated with antioxidative damage.
Insights
Astragaloside protects endothelial progenitor cells (EPCs) from oxidative damage caused by oxidized low-density lipoprotein (Ox-LDL). This compound enhances EPC proliferation and adhesion, suggesting a role in cardiovascular health through antioxidant mechanisms.
Area of Science:
- Cardiovascular Research
- Cell Biology
- Pharmacology
Background:
- Oxidative low-density lipoprotein (Ox-LDL) is implicated in endothelial progenitor cell (EPC) dysfunction.
- EPCs play a crucial role in vascular repair and maintaining endothelial health.
Purpose of the Study:
- To investigate the protective effects of astragaloside against Ox-LDL-induced oxidative damage in EPCs.
- To explore the underlying antioxidant mechanisms of astragaloside's action.
Main Methods:
- EPCs were isolated and exposed to Ox-LDL with or without varying concentrations of astragaloside.
- Cell proliferation and adhesion were assessed using MTT colorimetry and adhesion assays.
- Levels of superoxide dismutase (SOD) and malonaldehyde (MDA) were measured as indicators of oxidative stress.
Main Results:
- Ox-LDL significantly impaired EPC proliferation and adhesion, decreased SOD, and increased MDA.
- Astragaloside treatment dose-dependently improved EPC proliferation and adhesion.
- Astragaloside intervention notably increased SOD levels and reduced MDA content in Ox-LDL-damaged EPCs.
Conclusions:
- Astragaloside demonstrates significant protective effects on EPCs against Ox-LDL-induced oxidative damage.
- The protective mechanism of astragaloside appears to be associated with its antioxidant properties, mitigating oxidative stress.
