Related Experiment Video
Updated: May 30, 2026
Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
Published on: August 23, 2024
[Study on extraction and purification techniques and free radical scavenging activity of total flavonoids from
Chun-Yan Fu1, Yong-Hui Liu, Dai-Wu Chen
1Department of Pharmacy, Shaoyang Junior Medicine College, Shaoyang 422000, China. 2. Red Cross Hospital at Xinshao County of. chunyanfull@yahoo.com.cn
Objective:
To optimize the extraction and purification techniques of total flavonoids in Fissistigma oldhamii. The scavenging activity to DPPH of the total flavonoids and the extraction were studied in vitro.
Methods:
By orthogonal design, we investigated the effects of concentration of ethanol, temperature, ratio of solid to liquid and time of extraction on the total flavonoids extraction rate. The total flavonoids was separated and purified by AB-8 macroporous resin absorption.
Results:
The optimum extraction condition was that at extraction time of 3 h, concentration of alcohol 90%, solid/liquid ratio of 1:10 and temperature of 70 degrees C. The content of total flavonoids in the refined product was above 55%. The GFM-2 fraction showed antioxidant capacity in vitro.
Conclusion:
The optimum extraction condition and purification experiment are very useful for further development of industrial process. Meantime, the result may be helpful for further development of Fissistigma oldhamii as health protection foods.