Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute renal injury in ANCA-associated glomerulonephritis: etiology related to acute tubulointerstitial injury versus glomerular injury.

Renal failure·2026
Same author

Significance of urinary and serum neutrophil gelatinase-associated Lipocalin in Antineutrophil cytoplasmic antibody-associated Vasculitis with glomerulonephritis.

International immunopharmacology·2025
Same author

Serum ferritin is a superior biomarker for evaluating disease activity and kidney injury compared with C-reactive protein in anti-neutrophil cytoplasmic antibody-associated vasculitis.

Clinical rheumatology·2025
Same author

Morphology and Distribution of Fat Globules in Osteomyelitis on Magnetic Resonance Imaging.

Current medical imaging·2025
Same author

New insights into the stipitate hydnoid fungi Sarcodon, Hydnellum, and the formerly informally defined Neosarcodon, with emphasis on the edible species marketed in Southwest China.

IMA fungus·2024
Same author

Comparative Study on the Pharmacokinetics of Paeoniflorin, White Peony Root Water Extract, and Taohong Siwu Decoction After Oral Administration in Rats.

European journal of drug metabolism and pharmacokinetics·2023

Related Experiment Video

Updated: May 30, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
10:03

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

[Study on methods of deproteinization from Phellinus baumii polysaccharide].

Li-yuan Xie1, Yong Zhang, Wei-hong Peng

  • 1Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. xieliyuan77@163.com

Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
|August 10, 2011
PubMed
Summary
This summary is machine-generated.

The trichloroacetic acid (TCA) method is superior for removing protein from Phellinus baumii polysaccharide compared to the Sevag method. Optimal TCA deproteinization achieved 82% protein removal with minimal polysaccharide loss.

More Related Videos

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

Published on: March 12, 2010

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

Related Experiment Videos

Last Updated: May 30, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
10:03

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

Published on: March 12, 2010

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

Area of Science:

  • Biochemistry
  • Natural Product Chemistry
  • Pharmacognosy

Background:

  • Phellinus baumii polysaccharides exhibit potential biological activities.
  • Deproteinization is crucial for isolating and characterizing these polysaccharides.
  • Various methods exist for polysaccharide deproteinization, each with varying efficacy.

Purpose of the Study:

  • To compare the effectiveness of the Sevag and trichloroacetic acid (TCA) methods for deproteinizing Phellinus baumii polysaccharide.
  • To determine the optimal conditions for TCA deproteinization.
  • To evaluate the impact of deproteinization on polysaccharide yield.

Main Methods:

  • Crude Phellinus baumii polysaccharide was subjected to deproteinization using the Sevag method.
  • The TCA method was employed for deproteinization, with variations in treatment time, acid concentration, and repetition.
  • Protein removal efficiency and polysaccharide loss were quantified for each method and condition.

Main Results:

  • The TCA method demonstrated superior protein removal compared to the Sevag method.
  • Optimal conditions for TCA deproteinization were identified as 30 minutes treatment time, 5% trichloroacetic acid concentration, and three treatment cycles.
  • Under optimal conditions, the TCA method achieved 82% protein removal with a 10.8% polysaccharide loss.

Conclusions:

  • The TCA method is the preferred and most effective technique for deproteinizing Phellinus baumii polysaccharide.
  • Optimized TCA deproteinization balances efficient protein removal with acceptable polysaccharide yield.
  • This study provides valuable insights for the purification and further research of Phellinus baumii polysaccharides.