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Cloning and expression of the putative gene coding for GTP cyclohydrolase I from Escherichia coli
G Katzenmeier1, C Schmid, A Bacher
1Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Garching, F.R.G.
FEMS Microbiology Letters
|January 1, 1990
Abstract:
The putative gene coding for GTP cyclohydrolase I of Escherichia coli was isolated from a lambda gt11 expression vector library by using antibodies as a probe and has been subcloned on a 3.8 kb Bam HI fragment in the plasmid vector pUC13. E. coli cells carrying the recombinant plasmid designated pCYH express 100-fold increased levels of the enzyme. The protein formed under the control of the plasmid appears electrophoretically and immunochemically identical with the wild type enzyme.