Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration

Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration

Hemoperfusion and hemofiltration are critical techniques in medical treatments to eliminate accumulated drugs, metabolites, and electrolytes from the bloodstream. These methods are particularly vital in cases of accidental poisoning and drug overdose.Hemoperfusion involves passing blood through an adsorbent material to remove unwanted substances. The main adsorbents used in hemoperfusion include activated charcoal and Amberlite resins. Activated charcoal can adsorb both polar and nonpolar...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Downstream Processing

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

Chromatography: Introduction

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ethanol sclerotherapy or polidocanol sclerotherapy for symptomatic hepatic cysts.

United European gastroenterology journal·2018

Same author

[Liver support devices are no standard procedure in liver failure].

Deutsche medizinische Wochenschrift (1946)·2017

Same author

Quality of life 10 years after liver transplantation: The impact of graft histology.

World journal of transplantation·2017

Same author

Long-term outcome of liver transplant patients with Budd-Chiari syndrome secondary to myeloproliferative neoplasms.

Liver international : official journal of the International Association for the Study of the Liver·2015

Same author

Changes in liver stiffness using acoustic radiation force impulse imaging in patients with obstructive cholestasis and cholangitis.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2014

Same author

Increased HEV seroprevalence in patients with autoimmune hepatitis.

PloS one·2014

Search research articles

Related Experiment Video

Updated: May 30, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Fractionated plasma separation and adsorption: current practice and future options.

¹Department of Gastroenterology, Hepatology & Endocrinology, Hannover Medical School, Hannover, Germany. rifai.k@gmx.de

Liver International : Official Journal of the International Association for the Study of the Liver

|August 10, 2011

Summary

Fractionated plasma separation and adsorption (FPSA) liver support showed no overall survival benefit in acute-on-chronic liver failure patients. However, FPSA improved survival in specific subgroups, including those with hepatorenal syndrome or high MELD scores.

More Related Videos

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Related Experiment Videos

Last Updated: May 30, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Area of Science:

Hepatology
Artificial Liver Support Systems
Critical Care Medicine

Background:

Fractionated plasma separation and adsorption (FPSA) is an extracorporeal albumin dialysis method.
FPSA effectively removes protein-bound and water-soluble toxins without external albumin.
Previous studies suggest FPSA may be more effective than MARS for detoxification.

Purpose of the Study:

To evaluate the efficacy of FPSA therapy compared to standard medical treatment in patients with acute-on-chronic liver failure.
To assess survival rates at 1 and 3 months post-treatment.
To investigate potential survival benefits in specific patient subgroups.

Main Methods:

A large, randomized-controlled multicenter trial (HELIOS) was conducted.
145 patients with acute-on-chronic liver failure were included.
Comparison between FPSA therapy plus standard medical treatment versus standard medical treatment alone.

Main Results:

No statistically significant overall survival benefit was observed for FPSA therapy.
A predefined subgroup analysis revealed a significant survival benefit for patients with hepatorenal syndrome type I or MELD score >30.
FPSA therapy demonstrated safety, with an optimized citrate anticoagulation protocol.

Conclusions:

While FPSA did not improve overall survival in acute-on-chronic liver failure, it offers a significant survival advantage for specific high-risk subgroups.
FPSA shows promise in treating refractory cholestatic pruritus.
Further research is needed for acute liver failure applications.