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Related Concept Videos

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Related Experiment Video

Updated: May 30, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Published on: August 20, 2021

BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons.

Nabil-Fareed Alikhan1, Nicola K Petty, Nouri L Ben Zakour

  • 1Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

BMC Genomics
|August 10, 2011
PubMed
Summary

A new desktop application, BLAST Ring Image Generator (BRIG), allows for rapid visualization of hundreds of prokaryotic genome comparisons. This tool aids in determining genotypic differences and is user-friendly, requiring no scripting knowledge.

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Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Area of Science:

  • Genomics
  • Bioinformatics
  • Computational Biology

Background:

  • Visualizing prokaryotic genome comparisons is crucial for identifying genotypic differences.
  • Next-generation sequencing generates vast amounts of data, necessitating advanced visualization tools.
  • Existing tools often have limitations in the number of genomes compared, draft data utilization, or require scripting knowledge.

Purpose of the Study:

  • To develop a user-friendly, freely available desktop application for visualizing large-scale prokaryotic genome comparisons.
  • To enable rapid visualization of draft or complete genome data.
  • To overcome limitations of existing genome comparison visualization tools.

Main Methods:

  • Developed BLAST Ring Image Generator (BRIG), a cross-platform desktop application.
  • BRIG generates comparative genomic images with concentric rings representing sequence similarity.
  • The tool automatically handles file parsing and BLAST comparisons, requiring no specialist computational knowledge.

Main Results:

  • BRIG can visualize an unlimited number of prokaryotic genome comparisons in a single image.
  • Images display sequence similarity using colored concentric rings based on BLAST matches and percentage identity.
  • BRIG supports visualization of draft genome assembly information, including read coverage and breakpoints, and mapping of unassembled reads.

Conclusions:

  • BRIG addresses the need for a user-friendly tool for large-scale prokaryotic genome comparisons, especially with unfinished data.
  • The application provides an interactive graphical-user interface for generating comparative genomic images.
  • BRIG is freely available for all operating systems.