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Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
Yeon Suk Jung1, Reiko Kato, Toshie Tsuchiya
1Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
International Journal of Biomaterials
|August 10, 2011
Summary
The human cell line activation test (h-CLAT) assessed biodegradable polymers for skin sensitization. Several polymers increased CD54 expression, indicating varying allergenic potential.
Area of Science:
- Biomaterials Science
- Immunotoxicology
- Cellular Assays
Background:
- Developing non-animal testing methods for chemical and biomaterial skin sensitization is crucial.
- The human cell line activation test (h-CLAT) models dendritic cell (DC) functions for immune response assessment.
- Key DC markers like CD86, CD54, and MHC class II are indicators in skin sensitization testing.
Purpose of the Study:
- To evaluate the skin sensitization potential of various biodegradable polymers using the h-CLAT.
- To establish a relative ranking of the sensitizing abilities of different biodegradable polymers.
Main Methods:
- Utilized the human cell line activation test (h-CLAT) to assess biodegradable polymers.
- Monitored phenotypic changes in dendritic cells, specifically CD54 expression.
Main Results:
- Several biodegradable polymers demonstrated an increase in CD54 expression.
- The relative skin sensitization potential was ranked as PLLG (75:25) < PLLC (40:60) < PLGA (50:50) < PCG (50:50).
Conclusions:
- The h-CLAT effectively assessed the skin sensitization of biodegradable polymers.
- Findings may inform guidelines for biodegradable polymer use in medical scaffolds and allergenicity assessments.
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