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Related Experiment Video

Updated: May 30, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Particle separation in microfluidics using a switching ultrasonic field.

Yang Liu1, Kian-Meng Lim

  • 1Computational Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore 117576.

Lab on a Chip
|August 10, 2011
PubMed
Summary

This study introduces a novel ultrasound-assisted microfluidic technique for separating micro-sized particles. The method efficiently separates particles by size and acoustic properties using resonant acoustic modes.

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Area of Science:

  • Acoustofluidics
  • Microfluidics
  • Biotechnology

Background:

  • Microfluidic devices are essential for precise manipulation of small volumes.
  • Separation of micro-sized particles is critical in various scientific and industrial applications.
  • Acoustic manipulation offers a label-free method for particle separation.

Purpose of the Study:

  • To develop and demonstrate a new method for separating micro-sized particles using ultrasound in a microfluidic channel.
  • To investigate the separation of particles based on their size and acoustic contrast factors.
  • To achieve robust and efficient parallel-stream separation of different particle species.

Main Methods:

  • Utilizing ultrasound fields switched between the first and third resonant modes within a microfluidic channel.

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

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Last Updated: May 30, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
06:51

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

  • Employing initial hydrodynamic focusing to position particles near a side nodal line.
  • Conducting experiments with polystyrene microspheres of varying sizes to validate the method.

    • Main Results:

    • Successful separation of micro-sized constituents with positive acoustic contrast factors.
    • Demonstration of parallel-stream separation of two particle species with good robustness.
    • Validation of numerical simulations for optimizing the ultrasonic actuation and operating conditions.

    Conclusions:

    • The presented ultrasound-based method offers an efficient and robust approach for micro-particle separation in microfluidic devices.
    • The technique allows for size-dependent and acoustic contrast-dependent separation, enabling parallel-stream fractionation.
    • Hydrodynamic focusing and resonant acoustic modes are key to the effectiveness of this novel separation strategy.