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Related Experiment Videos

EndoCAM: a novel endothelial cell-cell adhesion molecule.

S M Albelda1, P D Oliver, L H Romer

  • 1Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.

The Journal of Cell Biology
|April 1, 1990
PubMed
Summary

Researchers identified endoCAM, an endothelial Cell Adhesion Molecule, crucial for cell-cell adhesion in vascular endothelium. This molecule is vital for regulating endothelial cell interactions and may influence blood cell adhesion.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • Cell-cell adhesion is mediated by cell surface molecules, including cadherins and cell adhesion molecules (CAMs).
  • These molecules are vital for tissue development and function.
  • Vascular endothelium, however, lacked well-characterized adhesion molecules until now.

Purpose of the Study:

  • To identify novel cell adhesion molecules in the vascular endothelium.
  • To characterize a newly discovered endothelial cell-cell adhesion molecule, designated endoCAM.
  • To investigate the role of endoCAM in endothelial cell adhesion and interactions.

Main Methods:

  • Identification and isolation of endoCAM from endothelial cells.
  • Characterization of endoCAM as a 130-kD glycoprotein.

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  • Immunological studies using antibodies against endoCAM.
  • Analysis of endoCAM's biochemical properties (serologic and protease susceptibility).

    • Main Results:

    • A novel cell surface molecule, endoCAM, was identified on endothelial cells in vitro and in situ.
    • EndoCAM is localized to the borders of contiguous endothelial cells and is also found on platelets and white blood cells.
    • Antibodies against endoCAM inhibited the initial formation of endothelial cell-cell contacts.
    • EndoCAM exhibits distinct serologic and protease susceptibility characteristics compared to known cadherins.

    Conclusions:

    • EndoCAM is a novel endothelial cell-cell adhesion molecule.
    • It plays a significant role in regulating endothelial cell adhesion processes.
    • EndoCAM may also be involved in interactions between endothelial cells and blood cells like platelets and white blood cells.