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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
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DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
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Published on: October 25, 2017

DNA-based soft phases.

Tommaso Bellini1, Roberto Cerbino, Giuliano Zanchetta

  • 1Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università degli Studi di Milano, Via F.lli Cervi 93, 20090 Milano, Italy. tommaso.bellini@unimi.it

Topics in Current Chemistry
|August 10, 2011
PubMed
Summary
This summary is machine-generated.

DNA molecules mediate interactions in self-assembling materials, enabling precise control over structure formation. This review covers DNA-based and hybrid systems, highlighting diverse emergent phases and hierarchical structuring for advanced nanotechnology applications.

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Area of Science:

  • Condensed matter physics
  • Nanotechnology
  • Material science

Background:

  • Recent advances in understanding DNA interactions have spurred the development of DNA-based self-assembled structures.
  • DNA-DNA interactions are crucial for the properties and assembly of these novel materials.
  • Exploiting programmable selectivity and tunable interaction strength of DNA is key to designing complex structures.

Purpose of the Study:

  • To review the state-of-the-art in molecular and colloidal systems interacting via DNA.
  • To discuss both fully DNA-constructed systems and hybrid systems incorporating colloidal particles.
  • To focus on systems where DNA-mediated interactions lead to emergent "phases" at large length scales.

Main Methods:

  • Review of existing literature on DNA-mediated self-assembly.
  • Analysis of systems involving DNA-coated colloidal particles (e.g., latex, metal).
  • Focus on self-assembly driven by DNA interactions, leading to phase formation.

Main Results:

  • DNA-mediated interactions enable the creation of diverse self-assembled structures.
  • Systems exhibit hierarchical structuring and phase formation over large length scales.
  • Tunable DNA interactions allow for a wide range of self-assembly patterns, including amorphous, liquid crystalline, and crystalline phases in 1D, 2D, and 3D.

Conclusions:

  • DNA-mediated self-assembly is a rapidly emerging field with significant potential in materials science and nanotechnology.
  • The ability to fine-tune DNA interactions offers unprecedented control over material structure and properties.
  • These systems pave the way for novel materials with applications in various scientific domains.