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Updated: May 30, 2026
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
Published on: March 13, 2013
[The CD133 polyclonal antibody generation and cancer stem cells identification]
Jun-Long Tian1, Pei-Ling Cai, Xiao-Qiang Xia
1State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Summary
Researchers developed a highly effective CD133 polyclonal antibody for cancer stem cell (CSC) research. This antibody accurately identifies CSCs in glioblastoma tissues and aids in CSC screening for mouse models.
Area of Science:
- Immunology
- Oncology
- Biotechnology
Background:
- Cancer stem cells (CSCs) are crucial for tumor growth and recurrence.
- Specific markers are needed for effective CSC identification and study.
Purpose of the Study:
- To generate a specific polyclonal antibody against CD133, a known CSC marker.
- To validate the antibody's utility in detecting cancer stem cells in glioblastoma.
Main Methods:
- Injected extracellular human CD133 peptide into rabbits to produce polyclonal antibodies.
- Utilized Western blot and immunohistochemistry for antibody validation.
- Tested antibody efficacy on glioblastoma (GBM) tissue samples.
Main Results:
- The generated CD133 antiserum effectively detected both overexpressed and endogenous CD133 via Western blot.
- Immunohistochemistry confirmed the antibody's ability to specifically label CSCs within GBM sections.
Conclusions:
- Successfully generated a highly efficient and specific CD133 polyclonal antibody.
- The antibody is suitable for labeling CSCs in tumor sections.
- The antibody can be used for CSC screening in mouse models.
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