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Detection of staphylococcal enterotoxin B. A comparative study of ELISA and ELIFA systems
H Dupont1, J Therasse, J M Pinon
1Centre d'Etudes du Bouchet, Vert-le-Petit, France.
Journal of Immunological Methods
|April 17, 1990
Abstract:
The enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunofiltration assay (ELIFA) sandwich methods have been compared for their ability to detect staphylococcal enterotoxin B (SEB) in biological samples. Using the avidin-biotin amplification system in the ELISA, high sensitivity was observed and staphylococcal protein A interference was completely prevented. In contrast, ELIFA was less sensitive than ELISA, but very easy to perform, fast and simple to read routinely.