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Related Experiment Videos

Lymphopoiesis and IL-7.

M B Widmer1, P J Morrissey, R G Goodwin

  • 1Immunex Research and Development Corporation, Seattle, Washington 98101.

International Journal of Cell Cloning
|January 1, 1990
PubMed
Summary
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Interleukin 7 (IL-7) is a cytokine that promotes the growth of early B lymphocyte cells. Research shows IL-7 also influences T lymphocyte activity, impacting both B and T cell behavior in vitro and in vivo.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Interleukin 7 (IL-7) was initially identified as a pre-B cell growth factor.
  • IL-7 influences the development and activity of both B and T lymphocyte lineages.
  • Long-term bone marrow cultures are crucial for studying lymphocyte development.

Purpose of the Study:

  • To discuss the effects of IL-7 on B and T lymphocyte behavior.
  • To describe the cloning and expression of the IL-7 gene.
  • To investigate IL-7's role in supporting lymphocyte growth.

Main Methods:

  • Utilized Whitlock-Witte long-term bone marrow cultures to isolate pre-B cells.
  • Employed direct expression cloning strategy to clone the IL-7 cDNA.
  • Screened COS 7 cells expressing IL-7 for their ability to support lymphocyte growth.

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Main Results:

  • IL-7 supports the stromal cell-independent growth of lymphoid elements.
  • IL-7 stimulates the growth of cells in fresh bone marrow cultures.
  • Enrichment for B220 antigen-expressing cells dramatically increased plating efficiency in IL-7.

Conclusions:

  • IL-7 is a key cytokine for B and T lymphocyte proliferation.
  • The cloning of IL-7 enabled further investigation into its biological functions.
  • IL-7 plays a significant role in hematopoiesis and immune cell development.