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Indirect Motor Pathways

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Related Experiment Video

Updated: May 30, 2026

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Published on: March 18, 2014

Motoneurons are essential for vascular pathfinding.

Amy H Lim¹, Arminda Suli, Karina Yaniv

¹Molecular Medicine Program, 15 N 2030 East, Room 4140, University of Utah, Salt Lake City, UT 84112, USA.

Development (Cambridge, England)

|August 11, 2011

Summary

This summary is machine-generated.

Zebrafish Netrin 1a guides motoneuron axons, which is crucial for blood vessel development. This study reveals how nerve guidance is essential for forming lymphatic vessels.

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A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

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Related Experiment Videos

Last Updated: May 30, 2026

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Published on: March 18, 2014

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Area of Science:

Developmental biology
Neuroscience
Vascular biology

Background:

Neural and vascular systems utilize shared guidance cues.
Netrin 1a and Dcc signaling pathways are implicated in cell guidance.

Purpose of the Study:

To investigate the role of Netrin 1a in coordinating neural and vascular development.
To elucidate the mechanism by which motoneuron axon guidance influences lymphangiogenesis.

Main Methods:

Zebrafish model system.
Netrin 1a and Dcc gene manipulation (morphants).
Laser ablation of motoneurons.
Cellular transplantation and mRNA overexpression.

Main Results:

Netrin 1a directs Dcc-mediated motoneuron axon guidance at the horizontal myoseptum.
Axon guidance by Netrin 1a is essential for parachordal chain (PAC) and thoracic duct formation.
Impaired PAC formation in netrin 1a or Dcc deficient zebrafish was rescued by interventions.

Conclusions:

Motoneuron axon guidance is a prerequisite for endothelial guidance and lymphangiogenesis.
This study provides in vivo evidence for axon-dependent vascular patterning.
Highlights the coordinated development of neural and vascular systems.