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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Related Experiment Video

Updated: May 30, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Published on: June 14, 2012

Genome engineering with zinc-finger nucleases.

Dana Carroll1

  • 1Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112-5650, USA. dana@biochem.utah.edu

Genetics
|August 11, 2011
PubMed
Summary

Zinc-finger nucleases (ZFNs) are powerful gene-targeting tools that create DNA breaks for precise gene editing. Ongoing advancements in ZFN design and understanding DNA repair mechanisms are expanding their potential in research and human gene therapy.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Zinc-finger nucleases (ZFNs) are engineered DNA-binding proteins fused to a nuclease domain, enabling targeted DNA cleavage.
  • ZFNs leverage cellular DNA repair pathways, such as non-homologous end joining and homology-directed repair, to achieve gene editing outcomes.

Purpose of the Study:

  • To review the historical development and diverse applications of ZFNs in genome editing across various organisms.
  • To highlight progress in ZFN design and selection methodologies.
  • To discuss the impact of DNA double-strand break repair mechanisms on ZFN efficacy and to explore future prospects, including human gene therapy.

Main Methods:

  • Review of existing literature on ZFN development and applications.
  • Analysis of ZFN-induced DNA repair processes and their outcomes (mutagenesis, gene replacement).

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Last Updated: May 30, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

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Published on: June 14, 2012

Mouse Genome Engineering Using Designer Nucleases
12:04

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  • Discussion of current challenges and future directions in ZFN technology.

    • Main Results:

    • ZFNs have been successfully applied for genome editing in numerous organisms and experimental systems.
    • Significant progress has been achieved in developing methods for designing zinc-finger arrays for novel genomic targets.
    • The efficiency and scope of ZFN applications are influenced by cellular DNA repair mechanisms, an area requiring further investigation.

    Conclusions:

    • ZFN technology has evolved into a versatile tool for precise genome editing.
    • Continued refinement of ZFN design and a deeper understanding of DNA repair pathways will enhance their utility.
    • ZFNs hold considerable promise for future applications, notably in the field of human gene therapy.