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Related Experiment Video

Updated: May 30, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Anionic solid lipid nanoparticles supported on protamine/DNA complexes.

Jiesheng Ye1, Aihua Wang, Chunxi Liu

  • 1School of Pharmaceutical Science, Shandong University, 44 Wenhua Xi Road, Ji'nan, People's Republic of China.

Nanotechnology
|August 11, 2011
PubMed
Summary
This summary is machine-generated.

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Novel anionic ternary nanoparticles were developed for gene delivery, showing improved DNA protection and transfection efficiency with reduced cytotoxicity compared to conventional methods.

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Gene Therapy

Background:

  • Gene delivery systems are crucial for therapeutic applications.
  • Conventional cationic vectors can exhibit toxicity and immunogenicity.
  • Developing safe and effective non-viral gene delivery vectors is essential.

Purpose of the Study:

  • To design and characterize novel anionic ternary nanoparticles for enhanced gene delivery.
  • To evaluate the protective effect of nanoparticles on DNA.
  • To assess the cytotoxicity and transfection efficiency of the developed nanoparticles.

Main Methods:

  • Fabrication of protamine/DNA binary complexes.
  • Preparation of anionic solid lipid nanoparticles (SLNs) via film dispersion-ultrasonication.

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Related Experiment Videos

Last Updated: May 30, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
08:19

Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications

Published on: November 17, 2015

  • Adsorption of anionic SLNs onto binary complexes to form ternary nanoparticles.
  • Characterization of size, surface charge, adsorption efficiency, DNA protection, cytotoxicity, and transfection efficiency.

    • Main Results:

    • Ternary nanoparticles exhibited a uniform size (approx. 258 nm) and negative surface charge (-17.16 mV).
    • High adsorption efficiency (96.75%) and effective protection of DNA from nuclease degradation were observed.
    • Lower cytotoxicity to A549 cells and comparable transfection efficiency to lipofectamine/DNA complexes were demonstrated.

    Conclusions:

    • Anionic ternary nanoparticles are effective non-viral gene delivery vectors.
    • These nanoparticles offer improved DNA protection and transfection efficiency with reduced toxicity.
    • The developed system shows potential for overcoming limitations of traditional cationic gene delivery vectors.