Related Experiment Video
Updated: May 30, 2026
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
Published on: October 19, 2015
Physical characterization methods for iron oxide contrast agents encapsulated within a targeted liposome-based
J A Dagata1, N Farkas, C L Dennis
1National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Nanotechnology
|August 11, 2011
Summary
This study uses scanning probe microscopy (SPM) to characterize intact liposome-based targeted nanoparticle delivery systems (NDS). This method allows for reliable assessment of nanoparticle size, stability, and function for improved drug delivery applications.
Area of Science:
- Nanotechnology
- Materials Science
- Biomedical Engineering
Background:
- Liposome-based nanoparticle delivery systems (NDS) are crucial for targeted drug delivery.
- Characterizing NDS in a native, fluid environment is essential for understanding their behavior.
- Non-selective binding can hinder accurate assessment of NDS properties.
Purpose of the Study:
- To immobilize and characterize intact liposome-based NDS using scanning probe microscopy (SPM) in a fluid environment.
- To determine the size, size distribution, functionality, and stability of NDS.
- To integrate SPM with other techniques for comprehensive NDS analysis.
Main Methods:
- Immobilization of intact liposome-based NDS via non-selective binding.
- Characterization using scanning probe microscopy (SPM) in a fluid imaging environment.
- Complementary analysis with superconducting quantum interference device (SQUID) magnetometry, dynamic light scattering, and electron microscopy.
Main Results:
- SPM successfully characterized intact NDS in a fluid environment.
- Determined size, size distribution, functionality, and stability of NDS containing super-paramagnetic iron oxide nanoparticles.
- Combined SPM data with other techniques for a multi-faceted analysis.
Conclusions:
- SPM provides a reliable method for assessing NDS properties in a fluid environment.
- Integration of SPM into NDS formulation and manufacturing enables assessment of size-dependent properties.
- This approach facilitates reliable and reproducible evaluation of the structure-function relationship in nanoscale entities.
More Related Videos
Related Concept Videos
01:27Bioavailability Enhancement: Drug Permeability Enhancement
After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
01:24Modified-Release Drug Delivery Systems: Site-Targeted
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.