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Updated: May 30, 2026
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Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Published on: May 9, 2020
Sequential expression of putative stem cell markers in gastric carcinogenesis
1Cancer Science Institute, National University Health System and Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
British Journal of Cancer
|August 11, 2011
Summary
Putative stem cell markers CD44, Musashi-1, and CD133 are elevated in gastric cancer. High CD44 and CD133 expression correlate with poorer survival and predict response to neoadjuvant chemotherapy.
Area of Science:
- Gastroenterology
- Oncology
- Biomarker Research
Background:
- Gastric carcinogenesis follows the Correa pathway.
- Putative stem cell markers (PSCs) like CD44, Musashi-1, and CD133 are implicated in cancer.
- Understanding PSC marker roles is crucial for gastric cancer management.
Purpose of the Study:
- Investigate CD44, Musashi-1, and CD133 expression in gastric carcinogenesis.
- Correlate PSC marker expression with prognosis and response to neoadjuvant chemotherapy.
Main Methods:
- Immunohistochemistry used on gastric cancer (GC) specimens across Correa pathway stages.
- Evaluated PSC marker expression before and after neoadjuvant chemotherapy (docetaxel, cisplatin, capecitabine - DCX).
Main Results:
- CD44, Musashi-1, and CD133 expression significantly increased in GC versus normal mucosa (P<0.001).
- CD44 and Musashi-1 elevated in premalignant lesions; CD133 expression localized to later-stage neoplastic tissues.
- High CD44 and CD133 expression linked to worse survival (P=0.014, P=0.019) and predicted pathological response to DCX chemotherapy.
Conclusions:
- CD44 and Musashi-1 are expressed in premalignant and invasive gastric lesions.
- CD133 expression is mainly confined to neoplastic gastric tissues.
- These PSC markers offer prognostic and predictive value in gastric cancer.
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