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Immunohistochemical localization of cyclic AMP-responsive element binding protein (CREB)-binding protein in the pig
Hanseul Oh1, Heechul Kim, Meejung Ahn
1Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University, Jeju, Korea.
Insights
Cyclic AMP-responsive element binding protein-binding protein (CBP) expression decreases in pig retinas postnatally. CBP plays roles in retinal cell homeostasis, neurite extension, and signal transduction.
Area of Science:
- Ophthalmology
- Neuroscience
- Cell Biology
Background:
- Cyclic AMP-responsive element binding protein-binding protein (CBP) is crucial for cellular processes.
- Understanding CBP's role in retinal development is essential for visual system research.
Purpose of the Study:
- To investigate the cellular localization and developmental changes of CBP expression in porcine retinas.
- To explore CBP's potential functions in different retinal cell types.
Main Methods:
- Western blot analysis was used to quantify CBP expression levels.
- Immunohistochemistry was employed to determine CBP's cellular and subcellular localization.
Main Results:
- CBP expression was high in 2-day-old piglets and significantly decreased in older pigs.
- CBP was localized in bipolar cells, Müller cells, and ganglion cells, with distinct changes during development.
- Species-specific differences in CBP expression were observed between pigs and rats.
Conclusions:
- CBP expression is developmentally regulated in the porcine retina.
- CBP is involved in Müller cell homeostasis, bipolar cell neurite extension, and photoreceptor signal transduction.
- CBP's differential expression highlights its varied roles in retinal cell function and species-specific adaptations.
Abstract:
This study evaluated the cellular localization of cyclic AMP-responsive element binding protein-binding protein (CBP) expression in pig retinas during postnatal development. Immunohistochemistry and Western blot analysis were performed on retinal tissue from 2-day-old, 5-week-old, and 6-month-old pigs. Western blot analysis detected the expression of CBP in the retinas of 2-day-old piglets and showed that it was significantly decreased in the retinas of 5-week-old and 6-month-old pigs. Immunohistochemically, CBP was intensely immunostained in protein kinase C alpha (PKCα)-positive-bipolar cells, glutamine synthetase-positive Müller cells, and in ganglion cells in 2-day-old piglets. CBP was detected weakly in the inner plexiform, outer nuclear, and rod and cone layers. CBP immunoreactivity in the ganglion cell layer was decreased in the retinas of 5-week-old and 6-month-old pigs, while clear CBP expression detected in the neurite of PKCα-positive bipolar cells in the inner nuclear layer. In addition, CBP immunoreactivity in Müller cells and glial fibrillary acidic protein-positive glial processes was particularly noteworthy in pig retinas, but not in rat retinas. The results indicate that CBP is expressed differentially in the retinal neurons and glial cells according to growth and animal species, and may play an important role in homeostasis in Müller cells, neurite extention in bipolar cells, and signal transduction in photoreceptor cells in the porcine retina.
