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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Dual fluorescence self-assembled multilayers bearing a fluorescent internal standard for interfacial sensing
Xiangying Sun1, Bin Liu, Zhifang Wang
1College of Material Science and Engineering, Huaqiao University, Xiamen, China. liumy@hqu.edu.cn
Summary
A new dual fluorescence sensor uses cadmium telluride (CdTe) quantum dots and acridine orange (AO) for highly accurate DNA detection. This sensor provides a stable internal reference signal, unaffected by environmental changes.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Developing sensitive and accurate biosensors is crucial for early disease detection and genetic analysis.
- Existing fluorescence-based sensors can be affected by environmental fluctuations, impacting reliability.
- Dual fluorescence systems offer potential for improved accuracy by incorporating an internal standard.
Purpose of the Study:
- To construct a novel dual fluorescence film sensor for sensitive and accurate DNA detection.
- To utilize layer-by-layer electrostatic attraction for sensor fabrication.
- To establish a reliable fluorescence-based assay with an internal standard for enhanced stability.
Main Methods:
- Fabrication of a multilayer film sensor (Quartz/PSS/AO/PSS/CS/CdTe) using layer-layer electrostatic attraction.
- Utilizing CdTe as the primary fluorescence probe and acridine orange (AO) as a shielded fluorescent internal standard.
- Measuring the ratio of dual fluorescence intensities to quantify DNA concentration.
Main Results:
- The sensor demonstrated high sensitivity and accuracy for DNA detection.
- Acridine orange (AO) fluorescence remained stable, serving as an effective internal standard.
- The dual fluorescence intensity ratio was independent of external factors like excitation intensity and detector voltage.
Conclusions:
- The developed dual fluorescence self-assembled multilayers sensor offers a robust platform for highly sensitive and accurate DNA quantification.
- The internal standard approach enhances sensor reliability by mitigating environmental interference.
- This technology holds promise for advanced diagnostic and analytical applications.
