Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

A collagen peptide-based physical hydrogel for cell encapsulation.

Charles M Rubert Pérez1, Alyssa Panitch, Jean Chmielewski

  • 1Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.

Macromolecular Bioscience
|August 11, 2011
PubMed
Summary

New collagen peptide hydrogels offer thermoresponsive properties for 3D cell growth. These materials provide ideal stiffness and pore structure, showing promise as scaffolds for human mesenchymal stem cells (hMSCs).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular and Antiangiogenic Effects of Paclitaxel-Loaded Nanoparticles: Influence of the Nanocarrier Type.

Molecular pharmaceutics·2026
Same author

Loading of therapeutic cell penetrating peptides into extracellular vesicles for pulmonary fibrosis.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Disturbed flow induces reprogramming of endothelial cells to immune-like and foam cells under hypercholesterolaemia during atherogenesis.

Cardiovascular research·2025
Same author

Chondrogenic and chondroprotective response of composite collagen I/II-hyaluronic acid scaffolds within an inflammatory osteoarthritic environment.

Biomaterials science·2025
Same author

Disturbed Flow Induces Reprogramming of Endothelial Cells to Immune-like and Foam Cells under Hypercholesterolemia during Atherogenesis.

Research square·2025
Same author

Disturbed Flow Induces Reprogramming of Endothelial Cells to Immune-like and Foam Cells under Hypercholesterolemia during Atherogenesis.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Collagen-based materials are crucial for regenerative medicine.
  • Developing advanced scaffolds is essential for 3D cell culture.
  • Thermoresponsive materials offer unique advantages in biomaterial applications.

Purpose of the Study:

  • To synthesize and characterize novel collagen peptide-based hydrogels.
  • To evaluate the thermoresponsive properties of these hydrogels.
  • To assess their suitability as scaffolds for human mesenchymal stem cell (hMSC) culture.

Main Methods:

  • Covalent conjugation of collagen peptides with 8-arm poly(ethylene glycol) star polymers.
  • Characterization of hydrogel viscoelasticity, thermal transition, and mechanical properties.

More Related Videos

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

Related Experiment Videos

Last Updated: May 30, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

  • Assessment of hydrogel microstructure and hMSC encapsulation and viability.

    • Main Results:

    • Successfully prepared collagen peptide-poly(ethylene glycol) hydrogels.
    • Demonstrated thermoresponsive behavior: liquid-like at elevated temperatures, elastic at room temperature.
    • Hydrogels exhibited suitable stiffness and a porous network supporting hMSC residence.

    Conclusions:

    • The developed hydrogels are thermoresponsive and possess favorable mechanical properties.
    • These collagen-based hydrogels show significant potential as scaffolds for 3D cell culture, particularly for hMSCs.
    • The material's tunable properties make it a promising candidate for tissue engineering applications.