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Updated: May 30, 2026
Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
Published on: November 28, 2014
A novel "by difference" method for assessing dermal absorption of polycyclic aromatic hydrocarbons from soil at
Richard P Moody1, Andrey V Tytchino, Anna Yip
1Exposure and Biomonitoring Division, Health Canada, Ottawa, Ontario, Canada. rick.moody@hc-sc.gc.ca
Abstract:
A highly precautionary cost-effective method for estimating dermal absorption using data from 24-h skin soap washes from in vitro dermal absorption tests in Bronaugh flow-through diffusion cells with human skin is reported. Skin was dosed with 16 U.S. Environmental Protection (EPA) priority polycyclic aromatic hydrocarbons (PAH) applied in mixture each at 2 μg/ml (ppm) in acetone without soil. Concurrent tests were conducted with an unspiked aqueous suspension of PAH-contaminated soil obtained from a Canadian federal contaminated site. Percentage dermal absorption was estimated "by difference" from the applied dose and that detected by high-performance liquid chromatography (HPLC) in 24-h skin soap washes. The dermal absorption for 11 PAH ranged from 71 to 88.3% without and with soil, respectively. Lower absorption was found for 5 PAH in soil, in the range of 26.4 to 60.8%. Data could not be corrected for evaporative loss due to inconsistent data from Tenax adsorbent. Corroboratory gas chromatography/mass spectroscopy (GC/MS) tests are needed. Previously published in vitro data from the authors' laboratory supported use of the "by difference" method.
