Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Unfolded Protein Response01:37

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
Acute Pancreatitis II: Pathophysiology01:21

Acute Pancreatitis II: Pathophysiology

The pathophysiology of acute pancreatitis centers on injury to pancreatic acinar cells, which initiates a cascade of harmful intracellular events.This injury leads to premature activation of trypsinogen to trypsin in the pancreas. Trypsin then activates other digestive enzymes, such as chymotrypsin, elastase, and phospholipase A2, which begin breaking down pancreatic tissue. The resulting autodigestion causes local inflammation, tissue swelling, hemorrhage, and fat necrosis.Injured acinar cells...
Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole Blackcurrant (Ribes nigrum) Alleviates High-Fat Diet Induced-Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice.

Molecular nutrition & food research·2026
Same author

Air quality prediction using multi-source remote sensing data integration with hybrid deep learning framework.

Scientific reports·2025
Same author

<i>Cheonggukjang</i>, a gut microbiota-modulating Korean fermented food, improves cholesterol and bile acid metabolism.

Food & nutrition research·2025
Same author

MicroarrayCancerNet: Hybrid optimized deep learning with integration of graph CNN with 1D-CNN for cancer classification framework using microarray and seq expression data.

Computational biology and chemistry·2025
Same author

Role of triage audit in an ongoing differentiated TB care initiative to reduce deaths in Tamil Nadu, India.

Public health action·2025
Same author

Anti-inflammatory effects of ethanolic extract from <i>Abeliophyllum distichum</i> (<i>Miseon</i> Tree) leaves in mice with dextran sulfate sodium-induced ulcerative colitis.

Food & nutrition research·2025

Related Experiment Video

Updated: May 30, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Spermidine may decrease ER stress in pancreatic beta cells and may reduce apoptosis via activating AMPK dependent

P B Tirupathi Pichiah1, U Suriyakalaa, S Kamalakkannan

  • 1Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, South Korea.

Medical Hypotheses
|August 12, 2011
PubMed
Summary

Spermidine may treat type 2 diabetes by activating autophagy and reducing endoplasmic reticulum stress. This preserves pancreatic beta cells, crucial for managing insulin resistance and diabetes.

More Related Videos

Isolated Pancreatic Islet Treatment and Apoptosis Measurement
09:36

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Published on: May 2, 2025

Related Experiment Videos

Last Updated: May 30, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Isolated Pancreatic Islet Treatment and Apoptosis Measurement
09:36

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Published on: May 2, 2025

Area of Science:

  • Biochemistry
  • Cell Biology
  • Endocrinology

Background:

  • Type 2 diabetes is linked to insulin resistance, primarily driven by endoplasmic reticulum (ER) stress.
  • ER stress triggers unfolded protein response, leading to pancreatic beta cell apoptosis and reduced cell mass.
  • Increasing BMI is associated with a higher risk of developing diabetes.

Purpose of the Study:

  • To investigate spermidine's potential as a therapeutic agent for type 2 diabetes.
  • To explore spermidine's role in modulating autophagy and ER stress pathways.
  • To determine if spermidine can preserve pancreatic beta cell function and mass.

Main Methods:

  • Review of existing studies on spermidine, autophagy, ER stress, and type 2 diabetes.
  • Analysis of spermidine's effects on autophagy induction via mTOR-dependent/independent pathways and AMPK activation.
  • Examination of spermidine's impact on ER stress reduction and beta cell apoptosis.

Main Results:

  • Spermidine promotes autophagy, a key pathway for clearing aggregated proteins.
  • Spermidine can reduce ER stress, thereby mitigating beta cell apoptosis.
  • Spermidine's dual action suggests a novel therapeutic approach for type 2 diabetes.

Conclusions:

  • Spermidine shows promise as a therapeutic candidate for type 2 diabetes by enhancing autophagy and reducing ER stress.
  • By preserving pancreatic beta cell mass, spermidine may effectively treat insulin resistance.
  • Further research into spermidine's mechanisms could lead to new diabetes management strategies.