Related Experiment Video
Updated: May 30, 2026
10:17
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
A steroidomic approach for biomarkers discovery in doping control
Julien Boccard1, Flavia Badoud, Elia Grata
1School of Pharmaceutical Sciences, University of Geneva and Lausanne, 20 Bd d'Yvoy, 1211 Geneva 4, Switzerland.
Forensic Science International
|August 12, 2011
Summary
This study introduces steroidomics for discovering new biomarkers to detect anabolic-androgenic steroid misuse. The approach enhances the athlete steroidal passport by identifying novel steroid conjugates for improved doping control.
Area of Science:
- * Analytical Chemistry
- * Biochemistry
- * Sports Science
Background:
- * The athlete steroidal passport (ASP) is crucial for detecting anabolic-androgenic steroid (AAS) misuse but is limited to known compounds.
- * Discovering new biomarkers is essential to enhance the effectiveness of AAS misuse detection.
- * Steroidomics offers a powerful untargeted approach for simultaneous analysis of numerous steroid compounds.
Purpose of the Study:
- * To discover novel biomarkers for oral testosterone undecanoate intake using an untargeted steroidomic approach.
- * To evaluate the potential of steroidomics in expanding the capabilities of the athlete steroidal passport.
- * To identify and validate new steroid metabolites for improved doping control.
Main Methods:
- * Utilized ultra-high-pressure liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS(E)) for untargeted steroidomic analysis of urine samples.
- * Applied automatic peak detection, mass-to-charge ratio filtering, and chemometric tools (N-way PLS-DA, O-PLS-DA, SUS-plot) for data analysis.
- * Employed receiver operating characteristic (ROC) curves to assess the specificity of identified biomarkers.
Main Results:
- * Confirmed the detection window for testosterone undecanoate intake through time-kinetic analysis.
- * Identified and evaluated both known and unknown steroid metabolites as potential biomarkers.
- * Highlighted the significance of monitoring both glucuronide and sulfate steroid conjugates.
Conclusions:
- * Steroidomics, particularly UHPLC-QTOF-MS(E), is highly effective for discovering new biomarkers in doping control.
- * The study validates the importance of including steroid conjugates in the athlete steroidal passport.
- * Promising novel biomarkers were identified, paving the way for enhanced anti-doping strategies.
Related Concept Videos
01:26Therapeutic Drug Monitoring: Drug Analysis Methods
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
01:29Pharmacogenomics: Identification of New Drug Targets
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
01:23Drug Concentrations: Measurements
Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
Plasma —...
Plasma —...
01:26Drug Discovery: Overview
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...