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Related Experiment Video

Updated: May 30, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

A newly discovered bacteriocin produced by Enterococcus faecalis 3915.

S G Dimov¹, S Peykov, D Raykova

¹Faculty of Biology, Department of Genetics, Sofia University 'St. Kliment Ohridski', Bulgaria. svetoslav@biofac.uni-sofia.bg

Beneficial Microbes

|August 12, 2011

Summary

Researchers identified Enterococcus faecalis 3915, a GRAS strain from Bulgarian cheese, producing a heat-stable bacteriocin, enterocin 3915. This bacteriocin shows potential for dairy applications due to its antimicrobial properties.

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Area of Science:

Microbiology
Food Science
Biochemistry

Background:

Enterococci are key in dairy fermentations, with some strains producing antimicrobial compounds.
Bacteriocins offer natural alternatives for food preservation and pathogen control.
The traditional Bulgarian cheese 'Kashkaval' utilizes specific starter cultures.

Purpose of the Study:

To characterize bacteriocin production by Enterococcus faecalis 3915 isolated from Kashkaval starter cultures.
To investigate the growth parameters, production kinetics, and preliminary properties of the bacteriocin, enterocin 3915.
To assess the potential application of enterocin 3915 in the dairy industry.

Main Methods:

Growth modeling using the logistic model.
Monitoring bacteriocin production kinetics.
Purification via ammonium sulfate precipitation and preparative PAGE.
Molecular mass determination and activity visualization using electrophoresis and agar overlay.

Main Results:

Enterococcus faecalis 3915 demonstrated constitutive bacteriocin synthesis with chromosomal gene localization.
Enterocin 3915 is a relatively heat-stable peptide with an approximate molecular mass of 6.5 kDa.
The producer strain is classified as Generally Recognized As Safe (GRAS).

Conclusions:

Enterocin 3915 exhibits inhibitory activity against commensals and pathogens.
The producer strain possesses potential probiotic properties.
Enterocin 3915 has promising applications in the dairy industry for natural preservation.