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Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
Histone methyltransferase SETD3 regulates muscle differentiation.
Gwang Hyeon Eom1, Kee-Beom Kim, Jin Hee Kim
1Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
The Journal of Biological Chemistry
|August 12, 2011
Summary
SET domain containing 3 (SETD3) is a novel histone methyltransferase that activates muscle-specific gene transcription. This protein plays a crucial role in muscle cell differentiation, promoting healthy muscle development.
Area of Science:
- Epigenetics and transcriptional regulation
- Molecular biology
- Muscle development and differentiation
Background:
- Histone lysine methylation is a key epigenetic mechanism regulating gene transcription.
- SET domain containing 3 (SETD3) is a newly identified protein with SET and Rubis-subs-bind domains.
Purpose of the Study:
- To investigate the function of SETD3 as a histone methyltransferase.
- To determine the role of SETD3 in muscle cell differentiation and transcriptional regulation.
Main Methods:
- Bioinformatic analysis to identify SETD3.
- Cloning of mouse SETD3.
- Overexpression and knockdown (shRNA) studies in muscle cells.
- Analysis of gene expression (myogenin, MCK, Myf6) and protein recruitment to gene promoters (MyoD).
Main Results:
- SETD3 functions as a histone H3K4 and H3K36 methyltransferase with transcriptional activation properties.
- SETD3 is highly expressed in muscle tissues and promotes the transcription of muscle-related genes.
- Overexpression of SETD3 enhances muscle cell differentiation, while its knockdown inhibits it.
- SETD3 is recruited to the myogenin gene promoter, cooperating with MyoD to activate transcription.
Conclusions:
- SETD3 is a H3K4/K36 methyltransferase essential for transcriptional regulation.
- SETD3 plays a significant role in promoting muscle cell differentiation through the activation of key muscle-specific genes.
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