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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: May 30, 2026

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

RNAi pathway integration in Caenorhabditis elegans development.

Sadegh Azimzadeh Jamalkandi¹, Ali Masoudi-Nejad

¹Laboratory of Systems Biology and Bioinformatics, Institute of Biochemistry and Biophysics and COE in Biomathematics, University of Tehran, Iran.

Functional & Integrative Genomics

|August 12, 2011

Summary

This summary is machine-generated.

Small RNAs regulate gene expression through RNA interference, impacting various cellular processes like gene silencing and defense in Caenorhabditis elegans. This review updates networks illustrating these crucial biological interactions.

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Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Small RNAs are crucial regulators of gene expression.
RNA silencing pathways are involved in diverse biological processes.
RNA interference (RNAi) is a key mechanism for gene regulation.

Purpose of the Study:

To review small RNA pathways in Caenorhabditis elegans.
To present an updated network of small RNA interactions with cellular mechanisms.
To highlight the role of small RNAs in various biological processes.

Main Methods:

Literature review of small RNA pathways.
Network analysis to illustrate interplay between small RNAs and cellular mechanisms.
Focus on Caenorhabditis elegans as a model organism.

Main Results:

Small RNAs are central to transcriptional and post-transcriptional gene regulation.
RNA silencing pathways connect apoptosis, meiosis, and antiviral defense.
RNA interference provides a rapid and energy-efficient gene regulation mechanism.

Conclusions:

Small RNAs play a fundamental role in coordinating diverse cellular functions.
The updated network provides a comprehensive overview of small RNA-mediated regulation.
Understanding these pathways is key to deciphering complex biological processes.