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Immunohistochemistry in lymphoproliferative diseases.

G Kelényi1

  • 1Department of Pathology, University Medical School, Pécs, Hungary.

Zentralblatt Fur Allgemeine Pathologie U. Pathologische Anatomie
|January 1, 1990
PubMed
Summary

Immunohistochemistry is crucial for diagnosing lymphoproliferative diseases, distinguishing reactive from malignant lesions and identifying lymphoma subtypes. This study highlights its utility with examples of B-cell chronic lymphocytic leukemia and a novel non-Hodgkin lymphoma.

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Area of Science:

  • Pathology
  • Immunology
  • Hematology

Background:

  • Immunohistochemistry is vital in diagnosing lymphoproliferative diseases.
  • It aids in determining lesion nature, cell origin, and lymphoma type, grade, and subtype.
  • Careful integration with routine pathomorphology is essential.

Purpose of the Study:

  • To demonstrate the diagnostic capabilities of immunohistochemistry in lymphoproliferative disorders.
  • To illustrate complex cases requiring detailed immunophenotypic analysis.
  • To investigate potential precursor relationships in novel lymphoma entities.

Main Methods:

  • Immunohistochemical staining techniques applied to lymph node biopsies.
  • Analysis of cell morphology and immunophenotype.
  • Correlation of immunohistochemical findings with routine histopathology.

Main Results:

  • Immunohistochemistry successfully differentiated reactive versus malignant lesions and identified specific lymphoma subtypes.
  • Case 1: Detailed analysis of B-cell chronic lymphocytic leukemia (CLL) cell origin and differentiation, noting follicular dendritic reticulum cells.
  • Case 2: Characterization of a new non-Hodgkin lymphoma (NHL) with intrasinusoidal B-cells, linked to monoclonal plasma cells, suggesting a precursor relationship.

Conclusions:

  • Immunohistochemistry is indispensable for accurate diagnosis and subclassification of lymphoproliferative diseases.
  • The study presents novel insights into CLL and a potential new NHL subtype.
  • Intrasinusoidal B-cells in the described NHL may represent precursors to plasma cells.

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