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Updated: May 30, 2026

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
Published on: December 24, 2015
The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration
Emanuela Frittoli1, Gianluca Matteoli, Andrea Palamidessi
1IFOM, FIRC Institute of Molecular Oncology Foundation at IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy.
Abstract:
Dendritic cells (DCs) flexibly adapt to different microenvironments by using diverse migration strategies that are ultimately dependent on the dynamics and structural organization of the actin cytoskeleton. Here, we have shown that DCs require the actin capping activity of the signaling adaptor Eps8 to polarize and to form elongated migratory protrusions. DCs from Eps8-deficient mice are impaired in directional and chemotactic migration in 3D in vitro and are delayed in reaching the draining lymph node (DLN) in vivo after inflammatory challenge. Hence, Eps8-deficient mice are unable to mount a contact hypersensitivity response. We have also shown that the DC migratory defect is cell autonomous and that Eps8 is required for the proper architectural organization of the actin meshwork and dynamics of cell protrusions. Yet, Eps8 is not necessary for antigen uptake, processing, and presentation. Thus, we have identified Eps8 as a unique actin capping protein specifically required for DC migration.
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