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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Updated: May 30, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

The microRNA body map: dissecting microRNA function through integrative genomics.

Pieter Mestdagh¹, Steve Lefever, Filip Pattyn

¹Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA. pieter.mestdagh@ugent.be

Nucleic Acids Research

|August 13, 2011

Summary

This study introduces a novel method to understand microRNA (miRNA) functions in specific tissues by integrating gene expression, target prediction, and network models. The findings are available on the interactive miRNA bodymap resource.

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

Genomics
Bioinformatics
Molecular Biology

Background:

Regulatory microRNA (miRNA) modules are increasingly linked to human disease and development.
Specific miRNA functions and their tissue-specific roles remain largely undefined.

Purpose of the Study:

To develop and present an innovative, multi-level approach for elucidating tissue-specific miRNA functions.
To create an accessible online resource for exploring and analyzing miRNA expression and function.

Main Methods:

Integrated analysis of miRNA and mRNA gene expression levels.
Incorporated miRNA and transcription factor target prediction.
Utilized mechanistic models of gene network regulation.
Experimental validation and comparison with published data.

Main Results:

A novel computational approach to predict miRNA functions was developed.
Predicted miRNA functions were validated experimentally or through literature comparison.
The miRNA bodymap, an interactive online compendium, was created to host these findings.
The resource allows for prioritization of miRNAs based on expression and function across tissues and diseases.

Conclusions:

The presented approach offers a comprehensive method to uncover tissue-specific miRNA functions.
The miRNA bodymap serves as a valuable, centralized resource for miRNA data mining.
This project has the potential to become a significant community resource for miRNA research.