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Related Experiment Videos

Variable expression of desmin in rat glomerular epithelial cells.

E Yaoita1, K Kawasaki, T Yamamoto

  • 1Department of Pathology, Niigata University School of Medicine, Japan.

The American Journal of Pathology
|April 1, 1990
PubMed
Summary

Desmin, a myogenic cell marker, is present in kidney glomerular epithelial cells (GECs). Its presence varies by rat strain and increases with age and kidney damage, suggesting a link to glomerular injury.

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Area of Science:

  • Nephrology
  • Cell Biology
  • Immunohistochemistry

Background:

  • Desmin is a known marker for myogenic cells.
  • Its presence in kidney glomerular epithelial cells (GECs) is not well-established.
  • Understanding desmin expression in GECs may offer insights into kidney health.

Purpose of the Study:

  • To investigate the presence and distribution of desmin in GECs across different rat strains.
  • To examine how desmin expression in GECs changes with aging and kidney damage.
  • To explore the potential correlation between desmin in GECs and glomerular epithelial damage.

Main Methods:

  • Immunofluorescence microscopy and immunoelectron microscopy were used.
  • Monoclonal and polyclonal anti-desmin antibodies were employed for staining.

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  • Four rat strains (SHR, Lewis, Fischer 344, WKA) were analyzed, including models of kidney injury.

    • Main Results:

    • Desmin was detected in GECs, with varying frequencies among rat strains.
    • WKA rats showed the highest desmin expression in GECs, while SHR rats had the lowest.
    • Desmin staining in GECs increased with aging and was significantly enhanced in aminonucleoside nephrosis, correlating with proteinuria.
    • Vimentin expression did not show similar heterogeneous distribution or changes.

    Conclusions:

    • Desmin is present in GECs, and its expression is heterogeneous across rat strains.
    • Aging and kidney damage, such as aminonucleoside nephrosis, lead to increased desmin expression in GECs.
    • The findings suggest a potential role for desmin in glomerular epithelial damage and kidney disease progression.