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Comparative study of anti-oncogenic microRNA-145 in canine and human malignant melanoma

Shunsuke Noguchi1, Takashi Mori, Yuki Hoshino

  • 1The United Graduate School of Veterinary Sciences, Gifu University, Japan.

The Journal of Veterinary Medical Science
|August 13, 2011
PubMed

Insights

MicroRNA-145 (miR-145) is downregulated in canine and human melanomas. Restoring miR-145 inhibits tumor growth and migration by targeting c-MYC and FASCIN1, indicating its tumor suppressor role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • MicroRNA-145 (miR-145) is frequently dysregulated in human cancers, impacting carcinogenesis and progression.
  • Its specific role in malignant melanoma, particularly in comparative contexts between species, requires further elucidation.

Purpose of the Study:

  • To investigate the function of miR-145 in canine and human malignant melanomas.
  • To determine the molecular mechanisms underlying miR-145's effects on melanoma cell growth and migration.

Main Methods:

  • Quantitative analysis of miR-145 expression in melanoma tissues and cell lines.
  • Ectopic expression of miR-145 in canine and human melanoma cell lines.
  • Assessment of cell growth and migration inhibition.
  • Target gene analysis (c-MYC, FASCIN1).

Main Results:

  • miR-145 was significantly downregulated in both canine and human melanoma samples.
  • Ectopic miR-145 expression inhibited melanoma cell proliferation.
  • miR-145 suppressed c-MYC in canine (LMeC) and human (A2058, Mewo) melanoma cells.
  • miR-145 suppressed FASCIN1, reducing cell migration in canine (KMeC) and human (A2058) melanoma cells.

Conclusions:

  • miR-145 functions as a tumor suppressor in both canine and human malignant melanomas.
  • The tumor-suppressive effects are mediated partly through the downregulation of c-MYC and FASCIN1.
  • These findings highlight miR-145 as a potential therapeutic target for melanoma treatment across species.

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