Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA-Programmed Amorphous PtCu Nanohybrids With Spatially Partitioned Functions for Hydrogen Evolution.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A multiple synergistic strategy to fabricate Si@TiO<sub>2</sub>/C-ZIF-8@CNFs with a hierarchical porous structure for enhancing Li storage kinetics.

Chemical communications (Cambridge, England)·2026
Same author

Molecular-Bridge-Directed Thermal Reconstruction of Semiconfined Fe-Based Sites in Dual-Carbon Matrix Enabling Long-Term Zinc-Air Batteries.

Inorganic chemistry·2026
Same author

Hierarchical dual-protection design in anti-chloride GO/Co<sub>3</sub>O<sub>4</sub>/Co<sub><i>x</i></sub>Ti<sub>4</sub>O<sub>9</sub>/TF for enhanced seawater oxidation electrocatalysis.

Chemical communications (Cambridge, England)·2026
Same author

La-doped high-entropy layered double hydroxides with reinforced active sites for ultra-stable oxygen evolution reaction.

Journal of colloid and interface science·2026
Same author

Sub-5 nm ZnCoS shells on Co cores <i>via</i> chemical vapor reconstruction: ultra-thin heterointerfaces enabling efficient oxygen electrocatalysis in zinc-air batteries.

Chemical communications (Cambridge, England)·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
Same journal

Vision-guided parallel manipulation of cells with optoelectronic tweezers.

Lab on a chip·2026
Same journal

Review of nanofluidic mass transport systems: engineering through physicochemical fields and interfacial properties.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

A high-performance microsystem for isolating circulating tumor cells.

Xiangjun Zheng1, Luthur Siu-Lun Cheung, Joyce A Schroeder

  • 1Department of Aerospace and Mechanical Engineering, the University of Arizona, Tucson, AZ, USA.

Lab on a Chip
|August 13, 2011
PubMed
Summary
This summary is machine-generated.

A novel microchannel flow field enhances circulating tumor cell isolation. This method improves specificity by selectively removing non-target cells, crucial for accurate cancer diagnostics.

More Related Videos

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

Related Experiment Videos

Last Updated: May 30, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Effective isolation of circulating tumor cells (CTCs) is vital for cancer diagnosis and treatment monitoring.
  • Current microfluidic systems often struggle to balance high target cell capture with efficient removal of non-target cells, impacting specificity.
  • Quantitative evaluation metrics like sensitivity and specificity are essential for comparing CTC isolation techniques.

Purpose of the Study:

  • To develop and evaluate a bio-functional microchannel system with a unique flow field pattern for enhanced CTC isolation.
  • To introduce and utilize sensitivity and specificity as key performance indicators for CTC isolation systems.
  • To demonstrate the effectiveness of a two-step flow field strategy in improving CTC separation efficiency.

Main Methods:

  • Utilized a bio-functional microchannel with a sequential slow and fast flow field pattern.
  • Employed antibody-functionalized microchannels targeting EpCAM or cadherin-11 receptors for cell capture.
  • Tested the system with homogeneous and binary mixtures of MDA-MB-231 (target) and BT-20 (non-target) cells at varying concentrations.

Main Results:

  • The two-step flow field significantly enhanced system specificity from approximately 0.85 to 0.95.
  • High system sensitivity, above 0.95, was maintained throughout the experiments.
  • Improved specificity was achieved even with a low target cell concentration (1:1000).

Conclusions:

  • The proposed two-step flow field pattern in bio-functional microchannels is highly effective for selective CTC isolation.
  • The developed system demonstrates superior performance in distinguishing and isolating target tumor cells from non-target cells.
  • This approach offers a promising advancement for sensitive and specific CTC detection in clinical applications.