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Fluorescence Correlation Spectroscopy and Fluorescence Recovery After Photobleaching to study receptor kinase
Mark Kwaaitaal1, Marieke Schor, Mark A Hink
1Laboratory of Biochemistry, Wageningen University, Wageningen, The Netherlands. markkw@mpiz-koeln.mpg.de
Abstract:
Plasma-membrane-localized receptor kinases are essential for cell-cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.
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