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Updated: May 30, 2026
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
Published on: February 25, 2013
[Neuregulin promotes spermatogonia proliferation in mice]
Ying-ji Jin1, Xiao-yan Pan, Yu-ji Jin
1Department of Dermatology, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, China.
Objective:
To explore the role of neuregulin (neural regulation of protein, NRG) in the process of mouse spermatogonia proliferation.
Methods:
Mouse testis fragments were cultured in the medium DMEM containing purified NRG1beta or NRG3 at the concentration of 50, 100 and 200 ng/ml, respectively, followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.
Results:
Compared with the control group, neuregulin significantly promoted the proliferation of spermatogonia (P < 0.05). The proliferation rates of spermatogonia cultured in the medium with 50, 100 and 200 ng/ml of NRG13 were 1.69, 1.55 and 1.86 times, and those with 50, 100 and 200 ng/ml of NRG3 were 1.35, 1.54 and 2.11 times that of the control.
Conclusion:
NRG1beta and NRG3 can promote the proliferation of mouse spermatogonia, and NRG is expected to be applied in the treatment of male infertility.
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