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[Multi-component quantitative method of Chinese herbal additives based on fingerprint]
Juanmin Luo1, Jibao Cai, Xue Xiao
1Technology Center of China Tobacco Jiangxi Industrial Co., Ltd., Nanchang 330096, China. greentea1106@163.com
Summary
A new High-Performance Liquid Chromatography (HPLC) method provides a full-quantified fingerprint for Chinese herbal additives. This validated technique ensures reliable quality control for herbal products.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Quality Control
Background:
- Chinese herbal additives require robust analytical methods for quality assurance.
- Existing methods may lack the precision and comprehensiveness needed for complex herbal matrices.
Purpose of the Study:
- To develop and validate a full-quantified fingerprint method for Chinese herbal additives using High-Performance Liquid Chromatography (HPLC).
- To establish a reliable analytical tool for the quality evaluation and control of herbal products.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) coupled with a Photodiode Array (PDA) detector.
- Utilized a Phenomenex Luna C18 column with gradient elution (acetonitrile-0.1% formic acid).
- Full spectrum scanning detection at a flow rate of 1 mL/min.
Main Results:
- The developed HPLC method demonstrated excellent precision, reproducibility, stability, and recovery.
- A comprehensive, full-quantified fingerprint of the Chinese herbal additive was successfully generated.
- The method allows for the simultaneous determination of seven key compounds.
Conclusions:
- The established HPLC method is simple, valid, and suitable for quality evaluation.
- This technique provides a robust approach for the quality control of Chinese herbal additives and their derived products.