Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancing Maturation of Human Neuromuscular Organoids via Electrical Stimulation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9.

bioRxiv : the preprint server for biology·2026

Same author

Seventh Åland Island Meeting on von Willebrand Disease.

Haemophilia : the official journal of the World Federation of Hemophilia·2026

Same author

Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.

Nano letters·2026

Same author

T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling.

The EMBO journal·2026

Same author

Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.

bioRxiv : the preprint server for biology·2026

Same journal

Higher-Order Clustering of Receptors Real-Time Projected by Plasmon-ruler on the Single Live Cell.

Nano letters·2026

Same journal

Achieving Fermi-Level Depinning and Ideal Metal Contact in <i>β</i>-Ga<sub>2</sub>O<sub>3</sub> Devices via MXene Integration.

Nano letters·2026

Same journal

AI-Assisted Electron Microscopy in Structure-Performance Analysis of Advanced Catalysts: From Atomic Resolution to Statistical Significance.

Nano letters·2026

Same journal

Electrically Switchable Ultraslow Dispersionless Polaritons via Twist Engineering in van der Waals Heterostructures.

Nano letters·2026

Same journal

Correction to "Ultrasonication-Triggered Ubiquitous Assembly of Magnetic Janus Amphiphilic Nanoparticles in Cancer Theranostic Applications".

Nano letters·2026

Same journal

Tunable Proximity Valley Splitting Via Interfacial Exchange Pinning in WSe<sub>2</sub>-CrBr<sub>3</sub>-CrPS<sub>4</sub> Heterostructures.

Nano letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 30, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

Binding-activated localization microscopy of DNA structures.

Ingmar Schoen¹, Jonas Ries, Enrico Klotzsch

¹Laboratory for Biologically Oriented Materials, ETH Zurich, Zurich, Switzerland. ingmar.schoen@mat.ethz.ch

|August 16, 2011

Summary

This summary is machine-generated.

We developed binding-activated localization microscopy (BALM) to visualize DNA organization. This superresolution technique achieves ~14 nm resolution by tracking individual dye binding events to double-stranded DNA.

More Related Videos

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Related Experiment Videos

Last Updated: May 30, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Area of Science:

Biophysics
Microscopy
Molecular Biology

Background:

Nucleic acid stains exhibit fluorescence enhancement upon binding to double-stranded DNA.
Superresolution microscopy requires precise localization of fluorescent signals.

Purpose of the Study:

To develop a superresolution microscopy technique based on the fluorescence enhancement of DNA-binding dyes.
To visualize the spatial organization of the bacterial chromosome with nanoscale resolution.

Main Methods:

Utilized a dynamic labeling scheme with bright fluorophores.
Applied binding-activated localization microscopy (BALM) to fixed Escherichia coli cells.
Achieved superresolution by localizing individual dye binding events to DNA.

Main Results:

Reached a resolution of approximately 14 nm (full width at half maximum).
Obtained a spatial sampling of 1/nm.
Successfully visualized the organization of the bacterial chromosome.

Conclusions:

Binding-activated localization microscopy (BALM) enables superresolution imaging of DNA.
The BALM principle is adaptable to other fluorescent dyes and molecular targets, including proteins.