Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Progress in research of multi-omics on risk for schizophrenia onset].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025

Same author

Uncovering PD-L1 among immune cell predictive biomarkers and actionable genetic alterations in anal squamous-cell carcinomas in the era of immunotherapy.

ESMO open·2025

Same author

Tobacco and alcohol use among lactating women and its association with child nutrition in India: findings from National Family Health Survey 2019-2021.

Public health·2024

Same author

[Research progresses of near-infrared autofluorescence imaging used in surgery of parathyroid glands].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2024

Same author

[Research on epidemiological characteristics of pneumonia and correlative factors of length of hospitalization in the elderly aged 60 years and older in Ningbo].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024

Same author

[Epidemiological characteristics and spatiotemporal distribution of schizophrenia in Ningbo, 2018-2022].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2023

Search research articles

Related Experiment Video

Updated: May 30, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Molecular imaging with nucleic acid aptamers.

H Hong¹, S Goel, Y Zhang

¹Department of Radiology, University of Wisconsin-Madison, 1111 Highland Ave, Madison, WI 53705-2275, USA.

Current Medicinal Chemistry

|August 16, 2011

Summary

This summary is machine-generated.

Aptamers offer versatile targeting for molecular imaging, enabling non-invasive disease detection and monitoring. Further development of aptamer-based imaging agents is crucial for clinical translation and patient benefit.

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Related Experiment Videos

Last Updated: May 30, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Area of Science:

Biomedical imaging
Molecular biology
Nanotechnology

Background:

Aptamers are synthetic nucleic acid ligands with desirable properties for molecular imaging.
They offer potential for non-invasive detection, stratification, and monitoring of diseases.

Purpose of the Study:

To review the current status of aptamer-based molecular imaging modalities.
To highlight the potential clinical applications of aptamer probes.

Main Methods:

Summarized fluorescence imaging (direct targeting and activatable probes).
Discussed molecular magnetic resonance imaging and ultrasound using aptamer agents.
Reviewed radionuclide-based imaging (SPECT, PET) and other techniques (CT, SPR, microscopy, SERS).
Highlighted the development of multimodality probes.

Main Results:

Aptamers have been successfully applied across various imaging modalities.
Multimodality probes enable concurrent imaging for comprehensive diagnostics.
Proof-of-principle studies demonstrate the potential of aptamer-based imaging.

Conclusions:

Aptamer-based molecular imaging shows significant promise for clinical applications.
Development of clinically translatable aptamer imaging agents is essential for patient benefit.