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Related Concept Videos

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: May 30, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Aptamer-based fluorescent biosensors.

R E Wang1, Y Zhang, J Cai

  • 1Department of Chemistry, Washington University in St. Louis, MO, USA.

Current Medicinal Chemistry
|August 16, 2011
PubMed
Summary
This summary is machine-generated.

Aptamers, selected through SELEX, are highly specific molecules ideal for biosensor development. This review focuses on aptamer-based fluorescent biosensors, highlighting their design and potential in disease diagnostics.

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Published on: May 28, 2012

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Aptamers are nucleic acid or peptide ligands selected using SELEX (Systematic Evolution of Ligands by Exponential Enrichment).
  • They exhibit high affinity and specificity for target molecules, making them valuable recognition elements.
  • Biosensors leverage aptamers for detecting various targets, with diverse detection techniques employed.

Purpose of the Study:

  • To review the development of aptamer-based fluorescent biosensors.
  • To emphasize their design strategies, sensitivity, and specificity.
  • To explore their potential applications in biomedical research and diagnostics.

Main Methods:

  • Categorization of aptamer-based fluorescent biosensors into labeled and label-free types.
  • Further classification into "signal-on" and "signal-off" sensor designs.
  • Summary of various detection techniques used in aptamer biosensors.

Main Results:

  • Aptamer-based fluorescent biosensors demonstrate high sensitivity and specificity.
  • Both labeled and label-free aptamer designs are effective.
  • "Signal-on" and "signal-off" strategies offer versatile detection mechanisms.

Conclusions:

  • Aptamer-based fluorescent biosensors show significant promise for biomedical research.
  • Their application in disease diagnostics is supported by promising results in biological samples like urine and serum.
  • Continued development in aptamer biosensor technology is crucial for advancing diagnostic capabilities.