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Related Experiment Video

Updated: May 30, 2026

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Published on: January 22, 2020

Exogenous glucose improves long-standing human sperm motility, viability, and mitochondrial function.

Alexandra Amaral¹, Carla Paiva, Marta Baptista

¹Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.

Fertility and Sterility

|August 16, 2011

Summary

Human sperm can survive over a week in phosphate-buffered saline (PBS) at room temperature. Adding glucose may double sperm viability, independent of mitochondrial activity.

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Area of Science:

Reproductive Biology
Sperm Physiology

Background:

Sperm preservation is crucial for assisted reproductive technologies and research.
Understanding factors influencing sperm viability at ambient temperatures is essential for developing improved storage methods.

Purpose of the Study:

To investigate the viability of human sperm in phosphate-buffered saline (PBS) at room temperature.
To determine the effect of glucose supplementation on sperm longevity in PBS.

Main Methods:

Human sperm samples were incubated in PBS at room temperature.
Viability was assessed over time.
The effect of glucose addition to PBS on sperm viability was evaluated.

Main Results:

A significant fraction of human sperm remained viable in PBS for over one week at room temperature.
Glucose supplementation potentially doubled the viability period of sperm in PBS.
The observed extended viability did not appear to be directly linked to sperm mitochondrial activity.

Conclusions:

Phosphate-buffered saline (PBS) can support human sperm viability for extended periods at room temperature.
Glucose may enhance sperm survival in PBS, offering a potential improvement for sperm storage.
The mechanism for glucose-mediated sperm preservation in PBS may not rely on mitochondrial respiration.