Combination gene therapy using VEGF-shRNA and fusion suicide gene yCDglyTK inhibits gastric carcinoma growth

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Ting Liu1, Ling Ye, Yongzheng He

  • 1Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha, Hunan Province, 410008, China.

Experimental and Molecular Pathology
|August 16, 2011
PubMed

Insights

This study combined suicide gene therapy with anti-angiogenesis gene therapy targeting vascular endothelial growth factor (VEGF) to improve cancer treatment. The combination therapy effectively reduced tumor growth and decreased microvessel density in xenografts.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Clinical trials of suicide gene therapy show limited success, necessitating improved therapeutic strategies.
  • Angiogenesis, regulated by vascular endothelial growth factor (VEGF), is critical for cancer progression.

Purpose of the Study:

  • To evaluate the anti-tumor effects of combining VEGF siRNA with the fusion suicide gene yCDglyTK.
  • To investigate the potential of a combined suicide and anti-angiogenesis gene therapy approach.

Main Methods:

  • A VEGF-targeted small hairpin RNA (shVEGF) was introduced into the CDTK/5-FC suicide gene system.
  • The combined gene therapy was tested in SGC7901 xenografts.

Main Results:

  • The combination therapy significantly delayed tumor growth in SGC7901 xenografts.
  • Introduction of shVEGF enhanced cell apoptosis and reduced microvessel density in xenograft tissue.

Conclusions:

  • The combination of suicide gene therapy and anti-angiogenesis gene therapy targeting VEGF shows potential for enhanced anti-tumor effects.
  • This strategy offers a promising new direction for cancer treatment by targeting both tumor cells and tumor vasculature.

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