Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

cAMP-dependent Protein Kinase Pathways

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

Regulation of Food Intake

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Therapeutic potential of dihydronicotinamide riboside (NRH) on obesity and glucose intolerance in mice.

Nature communications·2026

Same author

NAD<sup>+</sup> precursor supplementation in human ageing: clinical evidence and challenges.

Nature metabolism·2025

Same author

Emerging strategies, applications and challenges of targeting NAD<sup>+</sup> in the clinic.

Nature aging·2025

Same author

A human omentum-specific mesothelial-like stromal population inhibits adipogenesis through IGFBP2 secretion.

Cell metabolism·2024

Same author

Trigonelline is an NAD<sup>+</sup> precursor that improves muscle function during ageing and is reduced in human sarcopenia.

Nature metabolism·2024

Same author

Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair.

BMB reports·2024

Search research articles

Related Experiment Video

Updated: May 30, 2026

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Published on: May 16, 2021

Calorie restriction: is AMPK a key sensor and effector?

Carles Cantó¹, Johan Auwerx

¹Laboratory of Integrative and Systems Physiology and Nestle Chair in Energy Metabolism, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Physiology (Bethesda, Md.)

|August 16, 2011

Summary

This summary is machine-generated.

Dietary restriction extends lifespan by influencing nutrient sensing. This review explores how AMP-activated protein kinase (AMPK), an energy sensor, may mediate the health and longevity benefits of calorie restriction.

More Related Videos

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Published on: May 16, 2021

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

Area of Science:

Biochemistry
Molecular Biology
Gerontology

Background:

Dietary restriction is a known longevity intervention across diverse organisms.
Nutrient and energy sensing mechanisms are implicated in regulating lifespan.
AMP-activated protein kinase (AMPK) functions as a central cellular energy sensor.

Purpose of the Study:

To review the role of AMPK in mediating the health and lifespan benefits of calorie restriction.
To explore the molecular mechanisms by which AMPK influences metabolic adaptation and longevity.

Main Methods:

Literature review of studies on dietary restriction, calorie restriction, and AMPK.
Analysis of molecular pathways regulated by AMPK in response to energy availability.
Synthesis of evidence linking AMPK activity to healthspan and lifespan extension.

Main Results:

AMPK activation by calorie restriction promotes metabolic adaptation.
AMPK influences transcriptional reprogramming and cellular energy homeostasis.
Evidence suggests AMPK plays a crucial role in mediating the beneficial effects of dietary restriction.

Conclusions:

AMPK is a key molecular player in the longevity effects of dietary restriction.
Targeting AMPK pathways may offer therapeutic strategies for age-related diseases.
Further research is warranted to fully elucidate AMPK's role in aging and metabolism.