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Related Experiment Video

Updated: May 30, 2026

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

High-throughput yeast plasmid overexpression screen.

Michael S Fleming1, Aaron D Gitler

  • 1Neuroscience Graduate Group, University of Pennsylvania School of Medicine, USA.

Journal of Visualized Experiments : Jove
|August 16, 2011
PubMed
Summary
This summary is machine-generated.

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This study details a high-throughput yeast transformation protocol for plasmid overexpression screens. This method aids in identifying genes that modify the toxicity of disease-associated proteins in budding yeast (Saccharomyces cerevisiae).

Area of Science:

  • Cell Biology
  • Genetics
  • Biotechnology

Background:

  • Saccharomyces cerevisiae is a key model organism for studying fundamental cellular processes relevant to human disease.
  • Genetic screens in yeast have elucidated conserved eukaryotic cell biology, including the cell cycle and secretory pathways.
  • Recent advances include genome-wide yeast strain and plasmid libraries for systematic gene function analysis.

Purpose of the Study:

  • To present a detailed protocol for a high-throughput plasmid overexpression screen using budding yeast.
  • To demonstrate the application of this method in identifying genetic modifiers of protein aggregation-related toxicity.
  • To provide an adaptable methodology for studying other cellular phenotypes.

Main Methods:

  • Utilized a high-throughput yeast transformation protocol.

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Mating-based Overexpression Library Screening in Yeast

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Last Updated: May 30, 2026

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

  • Employed a liquid handling robot for efficiency.
  • Screened an arrayed library of 5,500 yeast plasmids for overexpression studies.

    • Main Results:

    • Successfully implemented a plasmid overexpression screen in Saccharomyces cerevisiae.
    • Identified genetic modifiers influencing the toxicity of aggregation-prone proteins.
    • Established a robust and adaptable protocol for genetic screening.

    Conclusions:

    • The presented high-throughput yeast transformation protocol enables efficient plasmid overexpression screening.
    • This methodology is valuable for dissecting genetic interactions and understanding disease mechanisms.
    • The protocol is readily adaptable for diverse cellular phenotype studies in yeast.