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Related Experiment Video

Updated: May 30, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Mining and validating grape (Vitis L.) ESTs to develop EST-SSR markers for genotyping and mapping.

Hong Huang, Jiang Lu, Zhongbo Ren

    Molecular Breeding : New Strategies in Plant Improvement
    |August 16, 2011
    PubMed
    Summary

    Grape expressed sequence tags (ESTs) provide a valuable new resource for developing simple sequence repeat (SSR) markers. These SSR markers are effective for grape genotyping and genetic mapping, aiding in understanding grape diversity.

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    Area of Science:

    • Plant Genomics
    • Bioinformatics
    • Molecular Biology

    Background:

    • Grapevine (Vitis) research benefits from robust genetic markers for genotyping and mapping.
    • Expressed Sequence Tags (ESTs) offer a rich source for developing functional markers.
    • Simple Sequence Repeats (SSRs) are highly polymorphic and widely used genetic markers.

    Purpose of the Study:

    • To develop and evaluate expressed sequence tag-derived simple sequence repeat (EST-SSR) markers for grapevine.
    • To assess the utility of these EST-SSR markers for genotyping and genetic mapping in different Vitis species and cultivars.

    Main Methods:

    • Computational pipeline for identifying and annotating SSRs in a large dataset of grape ESTs.
    • Clustering analysis to reduce redundancy and primer design for identified EST-SSRs.

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    Last Updated: May 30, 2026

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  • Polymerase Chain Reaction (PCR) amplification and analysis of polymorphism and heterozygosity in selected Vitis cultivars.

    • Main Results:

    • Identified 6,447 EST-SSR sequences from over 215,000 grape ESTs.
    • 1,037 EST-SSRs yielded successful primer designs, with 145 validated by PCR.
    • Polymorphism and heterozygosity analyses revealed marker utility in differentiating V. vinifera and V. rotundifolia cultivars.

    Conclusions:

    • EST-SSR markers are a valuable and efficient resource for grapevine genotyping and genetic mapping.
    • The developed markers demonstrate potential for distinguishing between grape cultivars and species.
    • This study provides a foundation for further genetic studies and marker-assisted selection in grape breeding.