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A study of Helicobacter pylori outer-membrane proteins (hom) A and B in Iraq and Turkey

Nawfal R Hussein1

  • 1Institute of Infection, Immunity and Inflammation and Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK. Nawfal.hussein@yahoo.com

Journal of Infection and Public Health
|August 17, 2011
PubMed

Insights

Helicobacter pylori outer-membrane protein genes (hom) were studied in Middle Eastern patients. The homA and homB genes showed no association with clinical outcomes, suggesting they may not predict disease in this region.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Genetics

Background:

  • Helicobacter pylori outer-membrane proteins (hom), particularly the homB gene, are potential virulence factors.
  • Previous research has not investigated the association between hom genes and clinical outcomes in Middle Eastern populations.

Purpose of the Study:

  • To investigate the prevalence of homA, homB, and cagA genes in H. pylori strains from Iraq and Turkey.
  • To determine the association between these genes and clinical outcomes in Middle Eastern patients.

Main Methods:

  • Gastric biopsies were collected from 134 H. pylori-positive patients (70 from Iraq, 64 from Turkey).
  • Polymerase Chain Reaction (PCR) was used to detect the presence of homA, homB, and cagA genes.

Main Results:

  • No significant association was found between homA or homB gene presence and clinical outcomes (peptic ulcer disease vs. non-peptic ulcer disease).
  • cagA positivity was significantly higher in patients with peptic ulcer disease compared to those with non-peptic ulcer disease (p=0.003, OR=3.6).
  • Two Iraqi strains exhibited intermediate-length PCR products for hom genes, requiring further functional investigation.

Conclusions:

  • The homA and homB genes may not be reliable indicators for predicting disease severity in H. pylori infections within the Middle East.
  • Further research is necessary to confirm these findings and elucidate the function of the intermediate-length hom genes.

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