Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genotype-first assessment of presentation and penetrance of neurofibromatosis type 1, autosomal dominant polycystic kidney disease, and Marfan syndrome within the All of Us research program cohort.

Genetics in medicine : official journal of the American College of Medical Genetics·2025
Same author

Lead Bullet Burden: Widespread Lead Exposure in Wild Carnivores and Unseen Consequences of Feeding Lead-Shot Meat to Captive Cheetahs (Acinonyx jubatus).

Journal of wildlife diseases·2025
Same author

Detection Dog Survey Detects African Wild Dog Presence and a Shared Marking Site.

Ecology and evolution·2025
Same author

Molecular and genetic characterization of sex-linked orange coat color in the domestic cat.

Current biology : CB·2025
Same author

Identifying Cryptic Mammals With Non-Invasive Methods: An Effective Molecular Species Identification Tool to Survey Southern African Terrestrial Carnivores.

Ecology and evolution·2025
Same author

EFFECTIVE ANTIBODY RESPONSE OF AFRICAN WILD DOGS (<i>LYCAON PICTUS</i>) TO CANINE DISTEMPER VACCINATION WITH A LIVE ATTENUATED VACCINE.

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians·2025
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Digital gene expression for non-model organisms.

Lewis Z Hong1, Jun Li, Anne Schmidt-Küntzel

  • 1Department of Genetics, Stanford University, Stanford, California 94305, USA.

Genome Research
|August 17, 2011
PubMed
Summary
This summary is machine-generated.

EcoP15I-tagged Digital Gene Expression (EDGE) profiles gene expression using short cDNA fragments, enabling accurate transcript quantification. This method excels in non-model organisms and detects subtle expression changes, outperforming RNA-seq for low-abundance transcripts.

More Related Videos

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
09:23

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Published on: August 16, 2017

Related Experiment Videos

Last Updated: May 30, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
09:23

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Published on: August 16, 2017

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) advances gene expression profiling but often requires high-quality reference genomes.
  • Existing methods face limitations in sensitivity and applicability to non-model organisms.

Purpose of the Study:

  • To introduce EcoP15I-tagged Digital Gene Expression (EDGE), a novel method for gene expression profiling.
  • To demonstrate EDGE's utility in quantifying transcript abundance, especially in organisms lacking annotated genomes.
  • To compare EDGE's performance against RNA-seq for gene expression analysis.

Main Methods:

  • EDGE utilizes ultra-high-throughput sequencing of 27-bp cDNA fragments tagged by EcoP15I.
  • Direct quantification of transcript abundance is achieved through unique gene tagging.
  • The method was applied to laboratory mice and cheetah skin biopsy samples.

Main Results:

  • EDGE can assay >99% of genes and reaches saturation with 6-8 million reads.
  • The method demonstrates minimal technical noise, a 10^6 dynamic range, and suitability for non-model organisms.
  • EDGE showed comparable relative transcript abundance to RNA-seq but improved detection of lowly expressed genes and lacked transcript length bias.
  • A mutation in Mc1r in mice was linked to reduced interferon response gene expression.
  • EDGE identified candidate genes influencing color variation in cheetahs.

Conclusions:

  • EDGE provides a robust and sensitive method for gene expression profiling across diverse organisms.
  • The technique is particularly valuable for transcriptomic studies in non-model organisms.
  • EDGE offers advantages over RNA-seq in detecting subtle expression differences and avoiding length bias.